![]() A comparison of primary protoplasmic and mixed fibrous/protoplasmic astroglial cultures. Alpha- and beta-adrenoceptor regulation of cyclic AMP accumulation in cultured rat astrocytes. Implications for the mechanism of action of selective phosphodiesterase inhibitors. The identification of a new cyclic nucleotide phosphodiesterase activity in human and guinea-pig cardiac ventricle. Differential modulation of tissue function and therapeutic potential of selective inhibitors of cyclic nucleotide phosphodiesterase isoenzymes. Role of intracellular Ca2+ sequestration in beta-adrenergic relaxation of a smooth muscle. Regulation of Ca2+-dependent K+-channel activity in tracheal myocytes by phosphorylation. Potassium currents in canine airway smooth muscle cells. Relaxation of human peripheral airway smooth muscle in vitro does not correlate with severity of chronic airflow limitation in vivo. de Jongste JC, Mons H, Bonta IL, Kerrebijn KF.Muscarinic cholinergic inhibition of adenylate cyclase in airway smooth muscle. Jones CA, Madison JM, Tom-Moy M, Brown JK.Role of low Km cyclic AMP phosphodiesterase inhibition in tracheal relaxation and bronchodilation in the guinea pig. Harris AL, Connell MJ, Ferguson EW, Wallace AM, Gordon RJ, Pagani ED, Silver PJ.Neuropeptide Y (NPY) receptors in HEL cells: comparison of binding and functional parameters for full and partial agonists and a non-peptide antagonist. Beta-adrenoceptor stimulation inhibits histamine-stimulated inositol phospholipid hydrolysis in bovine tracheal smooth muscle. Modulation of carbachol-induced inositol phosphate formation in bovine tracheal smooth muscle by cyclic AMP phosphodiesterase inhibitors. Modulation of fluoroaluminate-induced inositol phosphate formation by increases in tissue cyclic AMP content in bovine tracheal smooth muscle. Inhibition of histamine-stimulated inositol phospholipid hydrolysis by agents which increase cyclic AMP levels in bovine tracheal smooth muscle. ![]() Pharmacological studies with SK&F 94120, a novel positive inotropic agent with vasodilator activity. Gristwood RW, Eden RJ, Owen DA, Taylor EM.Relaxing actions of procaterol, a beta 2-adrenoceptor stimulant, on smooth muscle cells of the dog trachea. Fujiwara T, Sumimoto K, Itoh T, Suzuki H, Kuriyama H.Kinetic studies on the mechanism by which histamine H1 receptors potentiate cyclic AMP accumulation in guinea pig cerebral cortical slices. The pharmacology of a beta 2-selective adrenoceptor antagonist (ICI 118,551). Bilski AJ, Halliday SE, Fitzgerald JD, Wale JL.Links to PubMed are also available for Selected References. Get a printable copy (PDF file) of the complete article (1.2M), or click on a page image below to browse page by page. IBMX (1-100 microM) and low concentrations of rolipram (< 10 microM), also potentiated the response to 1 microM isoprenaline.(ABSTRACT TRUNCATED AT 250 WORDS) Full textįull text is available as a scanned copy of the original print version. ![]() The nonselective phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX) (0.1 mM) and the type IV, cyclic AMP selective, phosphodiesterase inhibitor rolipram (0.1 mM) both elevated basal -cyclic AMP levels by 1.8 and 1.5 fold respectively. A 1.6 fold (over basal) response was also observed following stimulation with NaF (10 mM). Forskolin (1 nM - 100 microM) induced concentration-dependent -cyclic AMP formation in these cells. Prostaglandin E2 also produced concentration-dependent -cyclic AMP formation (EC50 0.7 microM, response to 1 microM PGE2 6.4 fold over basal). However, with 50 nM ICI 118551 there was a reduction in the maximum response to isoprenaline. The response to isoprenaline was inhibited by ICI 118551 (10 nM), (apparent KA 1.9 x 10(9) M-1) indicating the probable involvement of a beta 2-adrenoceptor in this response in human cultured tracheal smooth muscle cells. After 10 min stimulation, 1 microM isoprenaline produced a 9.5 fold increase over basal -cyclic AMP levels. The response to 10 microM isoprenaline reached a maximum after 5-10 min stimulation and remained stable for periods of up to 1 h. Isoprenaline induced concentration-dependent -cyclic AMP formation with an EC50 of 0.2 microM. adenosine 3':5'-cyclic monophosphate (-cyclic AMP) responses were studied in primary cultures of human tracheal smooth muscle cells derived from explants of human trachealis muscle and in short term cultures of acutely dissociated trachealis cells. ![]()
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